Optimization of an Activity Assay of Coprinus Cinereus Peroxidase
نویسندگان
چکیده
منابع مشابه
Bisubstrate Kinetic Model for Enzymatic Decolorization of Reactive Black 5 by Coprinus cinereus Peroxidase
In this study, decolorization of the diazo dye, Reactive Black 5 (RB5) in a Coprinus cinereus peroxidase-catalyzed reaction has been investigated. A bisubstate kinetic model for the reaction rate based on the Ping-Pong mechanism was assumed for the enzymatic decolorization. Experiment...
متن کاملbisubstrate kinetic model for enzymatic decolorization of reactive black 5 by coprinus cinereus peroxidase
in this study, decolorization of the diazo dye, reactive black 5 (rb5) in a coprinus cinereus peroxidase-catalyzed reaction has been investigated. a bisubstate kinetic model for the reaction rate based on the ping-pong mechanism was assumed for the enzymatic decolorization. experimentswere conducted at different rb5 and hydrogen peroxideconcentrations in a batch mannerto estimate the intrinsic ...
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Kinetic analyses done with cell-free extracts of this basidiomycete fungus showed that the NADP-linked glutamate dehydrogenase exhibited positively co-operative interactions with the substrates 2-oxoglutarate and NADPH, negatively co-operative kinetics with NADP+ and was extremely sensitive to inhibition of deamination activity by ammonium and/or ammonia. The NAD-linked enzyme showed positive c...
متن کاملThe origin of multiple B mating specificities in Coprinus cinereus.
Mushrooms, such as Coprinus cinereus, possess large families of pheromones and G-protein-coupled receptors that are sequestered at the B mating-type locus and whose function is to confer vast numbers of different mating types. This ability results from complex patterns of cognate and noncognate pheromone/receptor pairings, which potentially offer a unique insight into the molecular interaction ...
متن کاملDe novo methylation of repeated sequences in Coprinus cinereus.
We have examined the stability of duplicated DNA sequences in the sexual phase of the life cycle of the basidiomycete fungus, Coprinus cinereus. We observed premeiotic de novo methylation in haploid nuclei containing either a triplication, a tandem duplication, or an ectopic duplication. Methylation changes were not observed in unique sequences. Repeated sequences underwent methylation changes ...
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ژورنال
عنوان ژورنال: Brazilian Archives of Biology and Technology
سال: 2018
ISSN: 1678-4324,1516-8913
DOI: 10.1590/1678-4324-2018160347